Leukocyte infiltration in multiple organs. Large infiltrates of hematopoietic cells were detected in liver, kidney, lung, and intestine sections of Ang-2 transgenic animals after 6 months of Ang-2 expression, as shown in H&E staining (A) and anti-CD45 immunohistochemistry (B). FACS analysis of dissociated lung samples showed a time-dependent increase of CD45⁺ leukocytes on prolonged Ang-2 expression (C), which was accompanied by a gain of CD45⁺ CD11b⁺ myeloid cells in the peripheral blood (D). Further analysis of leukocyte subsets of either WT (gray filled lines) or Ang-2 DT mice (red lines) showed a shift toward the myeloid lineage in the peripheral blood of transgenic mice at the expense of lymphocytes (E). Data are mean ± SEM. (C) n = 4. (D) n = 5. Scale bar represents 100 μm. *P < .05. **P < .01. ***P < .005.