Real-time PCR validation of VNN1 expression in different ITP groups and healthy controls. Five groups of samples were included in the validation: self-limited acute ITP (A; n = 8), chronic acute to chronic ITP during the acute phase (A-C; n = 7), healthy control (n, n = 5), resolved acute ITP (A-R; n = 6), and chronic ITP resistant to multiple treatments (RC; n = 6). The nonparametric Mann-Whitney 2-tailed test was performed in the statistical analysis. At the transcriptional level, VNN1 expression in the A-C group is significantly higher compared with the A (P = .0093), N (P = .0177), and A-R (P = .0221) groups; VNN1 expression in the RC group is significantly higher than in the A group (P = .0127). The upper and lower limits of each box represent the 75th and 25th percentiles, respectively; the horizontal lines inside the boxes represent medians; and the whiskers, extreme measurements.