Sox4 binds to the Sfpi1 URE. (A) A sequence alignment of the Tcf-binding sites and their surrounding areas in Sfpi1 UREs of indicated species. (B) ChIP assays performed using 32Dcl.3 cells with 6 different anti-Sox4 antibodies (lanes 3, 4, 5, 6, 7, and 10). The immunoprecipitated chromatin samples were analyzed by PCR using specific primers spanning the Tcf-binding site. (C) ChIP assays in U937 cells using anti-SOX4 and anti-TCF1antibodies. (D) Lack of SOX4-binding to a region located 600 bp upstream of the TCF-binding site.