Figure 4
Figure 4. Comparison between ChIP-chip and expression microarray datasets. (A) At day 8 of OP9 coculture, c-Kit+CD41+ cells were flow cytometrically isolated and used for microarray analyses of gene expression. (B) Venn diagrams showing the number of genes bound by HoxB4, the number of down-regulated genes (left) or up-regulated genes (right) on HoxB4 expression, and the overlap between the 2. (C) Graph showing the percentage of HoxB4-bound genes when the fold change threshold stringency of ChIP-chip enrichment is changed. The x-axis shows the ranking of genes in descending order in terms of the ChIP-chip score. Each number corresponds to fold enrichment values as follows: 1, 4.72; 20, 3.45; 30, 3.35; 40, 3.28; 50, 3.21; 60, 3.13; and 70, 3.11.

Comparison between ChIP-chip and expression microarray datasets. (A) At day 8 of OP9 coculture, c-Kit+CD41+ cells were flow cytometrically isolated and used for microarray analyses of gene expression. (B) Venn diagrams showing the number of genes bound by HoxB4, the number of down-regulated genes (left) or up-regulated genes (right) on HoxB4 expression, and the overlap between the 2. (C) Graph showing the percentage of HoxB4-bound genes when the fold change threshold stringency of ChIP-chip enrichment is changed. The x-axis shows the ranking of genes in descending order in terms of the ChIP-chip score. Each number corresponds to fold enrichment values as follows: 1, 4.72; 20, 3.45; 30, 3.35; 40, 3.28; 50, 3.21; 60, 3.13; and 70, 3.11.

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