Pathway analysis by IPA. (A) Significantly altered canonical pathways associated with chronic ITP compared with self-limited acute ITP. A total of 535 transcripts had a q value less than 5% by SAM analysis. These transcripts were mapped to 338 gene IDs in the IPA database and then analyzed by the IPA software to identify the most significantly perturbed canonical pathways. The canonical pathways included in this analysis are shown along the x-axis of the bar chart. The y-axis indicates the statistical significance on the left. Calculated using the right-tailed Fisher exact test, the P value indicates which biologic annotations are significantly associated with the input molecules relative to all functionally characterized mammalian molecules. The yellow threshold line represents the default significance cutoff at P = .05. (B) Significantly altered toxicity lists associated with chronic ITP. These lists have been grouped based on critical biologic processes and toxicologic responses. Only 5 toxicology lists reached statistical significance: PPAR, NF-κB, and oxidative stress pathways are predominant.