Functional immune responses in specific patients. (A) Deep skin biopsy of ≥ 5-cm area of induration (from patient MD012) showing CD8⁺/CD3⁺ T-cell infiltrate. (B) Tetramer analysis of T cells extracted from the skin biopsy depicted in panel A; 2.1% of the cells analyzed exhibited hTERT/survivin tetramer staining versus 0.1% in the control (tetramer negative) sample. (C) Proliferative response of CD8⁺ T cells (from patient UPCC/13406-11) after stimulation with hTERT/survivin peptide mix by CFSE dilution analysis; percentage of CFSE^dim was 11.2% in the hTERT/survivin-stimulated cells (bottom) versus 6.67% in cells stimulated with an irrelevant peptide derived from the Tax protein (top) and 6.2% in cells exposed to medium only (data not shown). (D) Proliferative response of CD8⁺ T cells from a second immunized patient (UPCC/13406-22) after stimulation with hTERT/survivin peptide mix by CFSE dilution analysis; percentage of CFSE^dim was 33.2% in the hTERT/survivin-stimulated cells (bottom) versus 11.7% in cells that were incubated in media only without peptide stimulation (top).