Angiogenic vessel derived VEGF-C regulates VEGF-A–induced lymphatic sprouting. (A) Double staining of corneal flat mounts for lymph- (LYVE-1) and angiogenesis (CD31) on day 4 after VEGF-A implantation. Arrowheads indicate descending lymphatic tips toward vascular vessels. Arrows indicate round tips of lymphatics over vascular plexus. Bar, 100 μm. (B) Confocal image illustrates descending lymphatic tips and lymphatics toward blood vessels on day 10 after VEGF-A implantation. Bar, 30 μm. (C-D) Lymphatic tip direction of VEGF-A, VEGF-C, or VEGF-A+VEGF-C mixture–induced lymphatics on day 6 (C). White symbols indicate tips toward GF pellet, and gray symbols indicate tips away from the pellet and percent of tips (D; n = 5-7). **P < .01.