Time course of VEGF-A–dependent corneal lymph- and angiogenesis. (A) Double staining of corneal flat mounts for lymph- (LYVE-1, red) and angiogenesis (CD31, green) in the front, side, and the back of the cornea. Bar, 200 μm. (B) Quantitative analysis of lymph- and angiogenesis on the indicated day after VEGF-A implantation (n = 4-8). (C) Quantitation of the number of LYVE-1(+) lymphatic vessels in corneas of PBS or VEGF-A–implanted animals (n = 4-6). (D) Ratio of the distance of lymphatic tips and vascular tips from limbal vessels in corneas of PBS or VEGF-A–implanted animals (n = 12-18). (E) Quantitation of the number of lymphatic vessels (n = 4-10). Bar, 200 μm. *P < .05; **P < .01.