VEGF-A–induced lymph- and angiogenesis. Pellets containing hVEGF (200 ng) or mVEGF-A (200 ng) were implanted into the corneas of C57BL/6 mice. Corneal lymph- and angiogenesis were examined on day 6 after implantation. (A) Microscopic pictures of corneal neovascularization. (B) Double staining of corneal flat mounts for angiogenesis (CD31) and lymphangiogenesis (LYVE-1). *Implanted pellets. Bar, 400 μm. (C) Quantitative analysis of vascular area (black) and lymphatic area (white; n = 4-8). (D) Blind round tips of lymphatics in normal corneas and branched sharp tips in VEGF-A–stimulated corneas of confocal images. Bar, 30 μm. *P < .05.