Expression of PML-RAR-α oncoprotein up-regulates cell surface expression of S100A10. PR9 and mock-transfected SN4 cells were treated with ZnSO4 for the indicated times. Cell lysates were prepared, and total levels of S100A10, annexin A2, α-enolase, and PML-RAR were examined by Western blotting with the use of actin as a loading control (A). Flow cytometric analysis of cell surface S100A10 (B) and annexin A2 (C). Quantification of flow cytometric analysis of cell surface S100A10 and annexin A2 in ZnSO4-treated PR9 and SN4 cells was calculated with the use of WinMDI software (**P < .01 and ***P < .001). Zinc-induced PR9 cells were incubated with uPA (50nM) and plasminogen (0.5μM), and the rate of plasmin generation was measured at 405 nm. Statistical analysis was performed by 1-way ANOVA with Tukey multiple comparisons, and data are expressed as the percentage of specific binding ± SD of 3 independent experiments (D).
