In vivo thrombosis and in vitro coagulation in STAT5-deficient and little intercrossed mice. (A) Control male (—; control/control), Lit male (gray —; lit^m/m/control), ST5 male (- - -; control/STAT5^−/−), and LitST5 male (gray - - -; lit^m/m/STAT5^−/−;) mice were injected with 3.0 μL/g 1:40 TF dilution in a PE model of thrombosis. Survival of male LitST5 was reduced compared with Lit and control male mice, but was similar to ST5. (B) Control female (—), Lit female (gray —), ST5 female (- - -), and LitST5 female (gray - - -) mice were injected with TF as in panel A. Survival of LitST5 female mice was reduced compared with Lit and control female mice, but was similar to ST5. (C) Whole blood was isolated from control male (●; control/control; n = 25), Lit male (○; n = 18), ST5 male (■; n = 9), and LitST5 male (□; n = 7) mice. TF-triggered whole blood clotting times were significantly shorter in LitST5 mice versus Lit, but were comparable to ST5. ***P < .001; 1-way ANOVA with Bonferroni post test. (D) Whole blood was isolated from control female (●; n = 28), Lit female (○; n = 5), ST5 female (■; n = 11), and LitST5 female (□; n = 8) mice. TF-triggered whole blood clotting times were significantly shorter in LitST5 mice versus Lit and control, but were comparable to ST5. *P < .05, ***P < .001; 1-way ANOVA with Bonferroni post test.