Expression of mature endothelial cell markers. (A) Relative expression of endothelial cell marker genes (VWF, P-SELECTIN, and ANG2). The samples were obtained at 3, 6, 9, 12 days of culture. The values are the mean ± SD of 3 independent experiments. A P value < .05 was considered to be statistically significant (*P < .05; n = 3). (B-C) Flow cytometric analysis for the endothelial markers CD31 and CD105 in hESC-derived (B) and hiPSC-derived (C) ECs.