Gating strategy for pErk, pS6, and pStat5 in CD34⁺, CD117⁺ blast cells. (A) A region used to exclude debris. (B) The circle represents the blast region defined by CD45 expression and side scatter. (C) The box shows the CD34⁺/CD117⁺ region. (D,G) pErk histograms at t = 0 (gray) or 2 (green) minutes for SCF and t = 0 (gray) or 8 (green) minutes for IL-3, respectively. (E,H) pS6 histograms at t = 0 (gray) or 4 (blue) minutes for SCF and t = 0 (gray) or 8 (blue) minutes for IL-3, respectively. (F,I) pStat5 histograms at t = 0 (gray) or 2 (red) minutes for SCF and t = 0 (gray) or 8 (red) minutes for IL-3, respectively. The histograms are representative of all the flow cytometry data reported here.