Adoptive transfer of γδ T-cell lymphoma to Rag1−/− recipients. γδ T-cell lymphomatous cells were isolated from the lymph nodes of Id3−/− mice and injected into Rag1−/− mice (1 × 107 per mouse). (A) Histopathological analysis of the indicated tissues from a representative mouse that received T-cell lymphoma 15 weeks ago (H&E staining, 5×; the inset in each slide was an enlarged area, 100×, n = 4 mice). Spl: spleen; Liv: liver; Lun: lungs; BM: bone marrow. Arrows indicate lymphoma cells. (B) Survival curve for recipient Rag1−/− mice (n = 8 mice) that received γδ T-cell lymphomatous cells from Id3−/− donors. Lymphomatous cells from 2 separate Id3−/− mice were transferred; all 8 Rag1−/− recipient mice died before 30 weeks. The data represent 2 independent experiments. (C-D). Flow cytometric analysis of γδ T-cell lymphoma isolated from the spleen (C) or other tissues (D) of Rag1−/− mice receiving γδ T-cell lymphoma cells 15 weeks ago. LN: lymph nodes; Liv: liver; Lun: lungs; BM: bone marrow. Data shown are dot plots of indicated markers on the lymphoma cells in a representative mouse of a total of 4 mice.
