Constitutively activated Akt (myrAkt) rescues the defects of ISV formation in vivo. (A) Constitutively activated myrAkt rescues the ISV defects caused by zNgBR MO3 injection at 24 hpf embryos. Fli1a-WISH staining (blue) was performed to localize the ISV at 24 hpf embryos. Top panels are the images of whole embryos at 24 hpf, and bottom panels are magnified images of the trunk region, showing WT (uninjected), MO3- (6 ng of zNgBR splice MO), MO3 + hNgBR- (6 ng of MO3 plus 100 pg hNgBR mRNA), MO3 + myrAkt-WT– (6 ng of MO3 plus 100 pg myrAkt), and MO3 + myrAkt-KD– (6 ng of MO3 plus 100 pg myrAkt-KD) injected embryos. Asterisk indicates location of ISV. Quantification of embryos with ISV defects (missing more than 5 ISV). (B) Constitutively activated myrAkt rescues the ISV defects caused by zNogo-B MO2 injection at 24 hpf embryos. Fli1a-WISH staining (blue) was performed to localize the ISV at 24 hpf embryos. Top panels are the images of whole embryos at 24 hpf, and bottom panels are magnified images of the trunk region, showing WT (uninjected), MO2- (6 ng of zNogo-B splice MO), MO2 + myrAkt-WT– (6 ng of MO2 plus 100 pg myrAkt), MO2 + hNogo-B– (6 ng of MO2 plus 100 pg human full-length Nogo-B mRNA), and MO2 + AmNogo-B– (6 ng of MO2 plus 100 pg human AmNogo-B) injected embryos. Asterisk indicates location of ISV. Quantification of embryos with ISV defects (missing more than 3 ISV). Anterior is to the left. myrAkt, myristoylated activated Akt/protein kinase B (PKB); myrAkt-KD, the kinase-dead version of myristoylated Akt/PKB.