Splenic CD127+ NK cells do not preferentially migrate into the LN. (A) Spleen cells from RAG1 KO mice were labeled with CFSE and intravenously injected (3-3.5 × 106 per mouse) into nonirradiated B6 mice. Blood, spleen, LNs, and BM were isolated 18 hours after injection and analyzed by flow cytometry for the presence of CFSE+ cells. Left bar graph shows the total numbers of CFSE+ cells recovered after 18 hours. Right bar graph shows the percentages of NK cells (NK1.1+CD49b+) among CFSE+ cells recovered in the indicated lymphoid organs. (B) Unlabeled RAG1 KO spleen cells were intravenously injected into nonirradiated B6.Pep3b mice, and after 18 hours lymphoid organs were analyzed by flow cytometer for the expression of CD45.2, NKp4, and CD127. The donor NK cells (CD45.2+NKp46+) and the host NK cells (CD45.2−NKp46+) were gated as shown by rectangles in the dot plots, and the expression of CD127 is shown in contour plots. Data are representative of ≥ 3 mice. The numbers in contour plots show the percentages of CD127+ cells (mean ± SD).
