Figure 5
![Figure 5. LPS-induced expression of IFN-β and IFN-λ1 is proportional to the number of κB sites. (A) Western blot analysis of the efficiency of siRNA-mediated RelA knockdown in HEK293-TLR4/CD14-Md2 cells using a range of siRelA concentrations (0.1hyphen]30nM). (B) Dose-dependent inhibition of IFNβ and IFN-λ1 mRNA expression in HEK293-TLR4/CD14-Md2 cells and stimulated with LPS (1 μg/mL). The x-axis shows percent RelA expression relative to control (100%) based on the densitometry analysis of Western blots. The y-axis is fold induction over mRNA expression in resting cells. Data shown are the mean + SD from a representative of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/25/10.1182_blood-2010-05-282285/4/zh89991062570005.jpeg?Expires=1722777895&Signature=z7YoSrHemZOcUc9mbw0jhw8kHKws~hP8QqB1fLcdcuRHT-nZE3-iqzNiK4kRseLJfjj45z9p7RvnbqIMG0beqbmkQ0sXUQyMrJTNlnwmhuON5ZFZOPNuWqktybmtt9ClcOMb66MbgWTKm4-KHJBDrMi85oOQNlGaONf~~D~9yZfZ7luROVU0KGZNR4esl20Ts3Mm6kzxPa3gryX4AEKclfvueB33WaTHL4VH5IUpIuCJFrxslIQNc5y-BZal9N1Rbjknove1chwLhLDx3OWz3STwKDSaCC7H45nPHDpfA2cf1KDo0EFPxuEx~9VFnQGGTRUjn96c3sTbaXl59PGEBA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
LPS-induced expression of IFN-β and IFN-λ1 is proportional to the number of κB sites. (A) Western blot analysis of the efficiency of siRNA-mediated RelA knockdown in HEK293-TLR4/CD14-Md2 cells using a range of siRelA concentrations (0.1hyphen]30nM). (B) Dose-dependent inhibition of IFNβ and IFN-λ1 mRNA expression in HEK293-TLR4/CD14-Md2 cells and stimulated with LPS (1 μg/mL). The x-axis shows percent RelA expression relative to control (100%) based on the densitometry analysis of Western blots. The y-axis is fold induction over mRNA expression in resting cells. Data shown are the mean + SD from a representative of 2 independent experiments.
