Egress of merozoites from adhering versus nonadhering iRBCs. (A) Snapshots of merozoite release when the iRBC is adhering to the substrate. (B) Maximum velocity of the successively ejected merozoites as a function of time in the case of no adhesion (○) or adhesion (●). T, H, B, and M are defined in the legend of Figure 4B. Inset, The initial position of the iRBC before opening and the merozoite dispersion after RBC membrane buckling in the case of no adhesion and adhesion of the iRBC to the glass substrate. Error bars represent the scale of velocity for each individual merozoite because of errors in determining the position of its center of mass. Scale bar, 3 μm.