C/EBP interrupts interaction between NF-κB p50 and HDAC. (A) 293T cells were cotransfected with the indicated combinations of 1 μg of CMV-p50, 3 μg of the indicated C/EBPα isoform (left) or C/EBPβ (right), and 1.5 μg of CMV-FLAG-HDAC1 or 750 ng of CMV-FLAG-HDAC3. Cell extracts were immunoprecipitated (IP) with the use of anti-FLAG beads, and the precipitated lysates or input extracts were Western blotted with the indicated antibodies. (B) 293T cells were transfected with similar quantities of C/EBPα, CMV-FLAG-HDAC1, or CMV-FLAG-HDAC3 as indicated, and cell extracts were immunoprecipitated with anti-FLAG beads followed by Western blotting with the use of the indicated antibodies. Representative blots from 3 experiments are shown. (C) 293T cells were cotransfected with 1 μg of CMV-p50, 1 μg of CMV-FLAG-p65, and 3 μg of the indicated CMV-C/EBP plasmid. Anti-FLAG beads were used to immunoprecipitate cell extracts, and the precipitated lysates or input extracts were subjected to Western blotting with the use of the indicated antibodies.