C/EBPα preferentially displaces HDAC from NF-κB p50. (A) A diagram depicting the H2K-Eμ–C/EBPα transgene. Total cellular extracts from BM-nucleated cells or splenocytes of WT or H2K-Eμ–C/EBPα transgenic (αTG) mice were subjected to Western blotting for C/EBPα or actin. (B) Single-cell suspensions of splenocytes from WT, WT;αTG, p50^−/−, or p50^−/−;αTG mice were subjected to ChIP analysis with the use of HDAC1 (left) or HDAC3 (right) antibodies, and enrichment of precipitated DNAs corresponding to the proximal or the distal regions of the Flip promoter were determined with quantitative real-time PCR relative to input. Averages from 3 repetitions are presented. (C) Flip transcripts were measured in splenocytes of WT or p50^−/− mice and normalized to mS16 levels with the use of quantitative reverse transcriptase PCR. Mean and SE from 3 independent experiments are shown.