MHC haplotype and amount of NKG2D ligand on BM grafts dictates NKG2D-dependent graft rejection. (A) C.B10 BM was transplanted into F1 recipients. (B) B10.D2 BM was transplanted into F1 recipients. Data are representative of 3 independent experiments. *P = .032 anti-NKG2D–treated versus control IgG–treated, and anti-NKG2D–treated group is significantly less than anti-Ly49D and Combo groups. Groups consisted of 4-5 mice treated with the reagents noted on the y-axis; “combo” refers to both anti-NKG2D and anti-Ly49D+Ly49A given simultaneously. Error bars represent SEM. Data shown are representative of 3 independent experiments.