Signal dysregulation in AML. Shown is an example of signaling dysregulation highlighting loss of response to SCF stimulation in the abnormal CD34⁺CD117⁺ cell population. The top 5 bivariate density plots show the representative kinetics of up-regulation of pAkt-A647 and pErk-A488 in the CD34⁺CD117⁺ cells from a normal BM in response to SCF stimulation. The bottom panel shows SCF stimulation of AML#5 demonstrating typical pAkt-A647 response but lack of pErk-A488 response. Acquired on Gallios flow cytometer and analyzed with FCS Express Version 3.