miR-34a functions as a tumor suppressor in AML with CEBPA mutations. (A) Quantitative real-time RT-PCR for miR-34a was carried out using bone marrow cells derived from AML patients. Values were normalized with U6. AML-NK, AML with normal karyotype; CB, cord blood; PB, peripheral blood. Data are represented as mean from 3 experiments. (B) Western blot analysis for E2F3 and E2F1 were carried out using bone marrow cells derived from AML patients. Values below the gel image indicate the E2F3 and E2F1 protein levels normalized to actin. (C) Growth curve of Kasumi-6 cells transfected with control or miR-34a.pCDNA vectors. Data are represented as mean ± SD from 3 independent experiments. (D) Flow cytometry of propidium iodide–stained Kasumi-6 cells transfected with control or miR-34a.pCDNA vectors from a representative experiment. (E) Cell-cycle profile of Kasumi-6 cells 2 days after transfection with control or miR-34a.pCDNA vectors. Data are represented as mean ± SD from 3 independent experiments. *P < .05, **P < .001.