Expression of LDOC1 and ZAP70 mRNAs measured by MF-QRT-PCR assay distinguishes between mutated and unmutated cases of CLL. (A) Dot-plot for LDOC1 mRNA expression. For the mutated cases, 65 of 67 mutated cases were unambiguously negative for LDOC1 mRNA (higher ΔΔCt values) and 2 were positive (lower ΔΔCt values). For the unmutated cases, 43 of 63 unmutated cases were positive for LDOC1 mRNA and 20 were negative. (B) Dot-plot for ZAP70 mRNA expression. For the unmutated cases, 53 of 63 unmutated cases were positive for ZAP70 mRNA and 10 were negative. Eleven of 67 mutated cases were positive for ZAP70 mRNA and 56 were negative.