isomiR pattern analysis. (A) miR-20a isomiRs. The reference sequence for miR-20a is shown with the mature miRNA bolded. Lower case letters represent nucleotide deviations from reference. The percentage of each miR-20a isomiR relative to the total sequence reads for miR-20a in the AML1 and CD34⁺ cells samples is shown. (B) The percentage of each isomiR relative to the total read counts for a specific miRNA were calculated for all miRNAs that had at least 1000 read counts between the AML1 and CD34 patient samples.