miR-223 expression in AML. (A) The relative expression of miR-223 in AML1 compared with the total expression of all miRNAs based on the Agilent array (array) and RNA sequencing (deep sequencing) data are shown. (B) The relative expression of miR-223 compared with RNU48 was determined using real time RT-PCR on undiluted RNA from AML1 and RNA diluted 10-fold (1/10) or 100-fold (1/100) with water. (C) miR-223 expression relative to RNU48 in 27 AML samples and 8 CD34 samples from healthy donors is shown. AML1 is indicated by the arrow. The dashed line indicates 2 SDs above the mean using data from the CD34 samples.