Phenotypic analysis of mice expressing a mutated (C294Y) hepatocyte-specific Hfe transgene (Hfe^C294Y tg). Schematic drawing of the Hfe^C294Y tg construct (A). (B-C) Box plots depicting the measurement of serum transferrin saturation (%; B), and non-heme liver iron (μg/g wet weight; C). WT (n = 7), Hfe^−/− (n = 13) and Hfe^−/−Hfe^C294Y tg (n = 16), are depicted in box plots as in Figure 1.