Analysis of BMP signaling in Hfe^−/− mice expressing hepatocyte-specific Hfe transgenes. Total mRNA was harvested from wild-type (WT), Hfe^−/−, Hfe^−/− Hfe-full length transgene (Hfe^WT) or Hfe^−/−Hfe-cMyc transgenic (Hfe^ΔCD−MYC tg) livers (n = 5 for each genotype) and hepcidin (Hamp; A), Id1 (B), and Bmp6 (C) mRNA was assessed by quantitative real-time PCR, normalized to β-actin (Actb), and then expressed relative to the WT value whose mean was defined as 1.0. Ratios are expressed ± SEM.