ROS levels, apoptosis, and cytokine response in Fancd2^−/− and wild-type bone marrow cells. (A) Fluorescence intensity in KSL subsets of Fancd2^−/− and wild-type bone marrow after carboxy-H2DCFDA staining for intracellular ROS. Oxidation of carboxy-H2DCFDA by ROS generated fluorescence detectable by flow cytometry. (B) Quantification of early apoptotic KSL cells after 7-AAD, annexin V, and KSL staining. Data represent 7 mice for each group. NS denotes not significant. (C) Resveratrol suppressed R848-induced TNF-α overproduction in Fancc-shRNA–treated THP1 human leukemia cells. The experiments were done in triplicates on a 96-well plate.