Hematopoietic defects in Fancd2^−/− mice. (A) In vivo competitive repopulation of mixed FA (Fancc^−/− or Fancd2^−/−) and ROSA26^Tg/O bone marrow cells. Quantitative real-time PCR (qPCR) analyses were performed to evaluate donor contribution to the peripheral blood cells from each donor (7 or 9 months posttransplantation for Fancd2^−/− or Fancc^−/− donors, respectively). Three independent qPCR analyses were performed for each sample, and results from 5 animals were pooled together for each experimental group. P values were calculated by the 2-tailed, paired Student t test. Error bars represent SEM. (B) Representative picture for CAFC assay. The arrow indicates cobblestone colony. The image was acquired on an Axiovert 200 inverted microscope with an AxioCam MRc color camera at room temperature using AxioVision Release 4.8 software (Carl Zeiss MicroImaging). Original magnification ×100 (with 10× objective lens). (C) Quantification of CAFC results. P values were calculated by 2-tailed, paired Student t test.