Dexamethasone priming enhances CD163/HO-1–mediated Hb clearance and detoxification. Comparative proteome analyses of untreated and Hb:Hp-treated monocytes that were primed with dexamethasone (red signatures) or unprimed (black signatures). (A) All 1034 proteins were identified by more than 2 peptides. Each signature (bar) represents the maximum and minimum protein ratios (ie, [protein XYZ in Hb:Hp + dexamethasone cells]/[protein XYZ in control cells]). Proteins were sorted along the x-axis according to their expression level in monocytes treated with both dexamethasone and Hb:Hp. To allow direct comparison of the expression changes of each protein, the corresponding protein values for cells treated with Hb:Hp alone (ie, without dexamethasone priming) are shown at the same position on the x-axis. The difference between the 2 ratios (the space between the red and black symbols) can be attributed to the dexamethasone priming effect. (B) The top 25 up-regulated and top 25 down-regulated proteins from Figure 1A. A complete list of proteins, including identifications and scores, is provided in supplemental Table 2. Black/red signatures indicate proteins found in all 3 independent experiments. Gray/orange signatures indicate proteins identified in 2 experiments.