Marginal zone B-cell loss in il2ra^−/− mice is restored in DKO mice. Analysis of B-cell populations in the spleen (A-C) and peritoneum (D) of 6- to 8-week-old mice. (A) Percentages (left panel) and total numbers (right panel) of CD19⁺ B cells. (B) Percentages of CD21⁺CD23⁻ MZ B cells among total CD19⁺ B cells (left panel) and total number of MZ B cells (right panel). Symbols represent individual mice and values in column plots indicate averages ± SEM of groups of mice (n = 4-5/group). (C) Dot plots of CD21 and CD23 expression gated on CD19⁺ B cells of individuals representative for the group. Data are representative of at least 3 independent experiments. (D) Percentages of IgM^hi CD23⁻ CD5⁺ B1a cells and IgM^hi CD23⁻ CD5⁻ B1b cells (left panel) and total B1 cell numbers (right panel) in the peritoneal cavity. Three mice per group were analyzed.