Figure 7
![Figure 7. Impact of MTX concentration and pH on the activity of PCFT D109 mutants. (A) [3H]-MTX influx (10μM) over 1 minute at pH 5.5. (B) [3H]-MTX influx (100μM) at pH 5.5. (C) [3H]-MTX influx (0.5μM) at pH 4.5. (D) [3H]-MTX influx (100μM) at pH 4.5.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410007.jpeg?Expires=1723148639&Signature=oki7PzTQlkiT2I85wk5acH7sCL~tqbGDnTbv9y9Tqf~2t4NWodssMTILhxeELePcFlv57ZTYxGRirxx5fpiJx5Ikug-DQaXb5T7ECi8jO6yuP-cuP7FEZgxEndYGcNE6knyn-LXBUsuqRIm2M7994Cd1OLvD-c6f~0CKuC5z4KoQ6aaKX0oLne~m8AzB4BB6HgrJgVBbzwsk~eE0fP2tCb22WGz-p5xge4idtSFCgufiw2kr0VJ7ndpKOQLiMUgeDD69aBMbSvzRxg-gdkvuf7GniqxQC6TPWxrviLzPEA1XfGJsgXfvZyzwrAo7AX2L4B5fyck038tn~PVU7u7C-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impact of MTX concentration and pH on the activity of PCFT D109 mutants. (A) [3H]-MTX influx (10μM) over 1 minute at pH 5.5. (B) [3H]-MTX influx (100μM) at pH 5.5. (C) [3H]-MTX influx (0.5μM) at pH 4.5. (D) [3H]-MTX influx (100μM) at pH 4.5.
