Figure 6
![Figure 6. Functional properties of D109 PCFT mutants. (A) [3H]-MTX influx (0.5μM) over 1 minute at pH 5.5. Data are the mean ± SEM from 3 independent experiments. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein (middle panel) of D109 PCFT mutants. β-actin was the loading control (bottom panel). The graph shown is a representative of 2 independent experiments. The vertical line between the D109S and Mock lanes indicates repositioned gel lanes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410006.jpeg?Expires=1724916955&Signature=3wOfV1KytRjI~ajmMk6P132z8AZbJw9pJOJmcUD0XGFo8a0aF1iyEPRRYCkhDhT1dxBt0kfksgl8AEKpIcMLdHjs78Wo2ZrbAhjkb7jRQVRDBdT7KQf~Rkrsq4xaIKbHCCr-hakXCPWe~I4QeJpHFI9XzDQINgoeGmmfTTaF-gMusoH3geyjTd3~~vnp4ra9AFTOsJziA3X1XSjdeLz~yn6CrrZrq98g5jhraF-UHb6ywFWlxCZZqn64pkdQ0AsMFKX8rUYk2FmExB1EvURGKDBXOK~XRV2RLGBUyu~GghdTktkv46uJB0~9HCNwH2zXkwKTbyqzSgdHvOVr~n~gGw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional properties of D109 PCFT mutants. (A) [3H]-MTX influx (0.5μM) over 1 minute at pH 5.5. Data are the mean ± SEM from 3 independent experiments. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein (middle panel) of D109 PCFT mutants. β-actin was the loading control (bottom panel). The graph shown is a representative of 2 independent experiments. The vertical line between the D109S and Mock lanes indicates repositioned gel lanes.
