Figure 4
![Figure 4. Expression and function of D156 PCFT mutants. (A) [3H]-MTX influx was performed at 0.5μM and pH 5.5 over 1 minute. Data are the mean ± SEM from 3 independent experiments performed on different days. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein at the cell surface (middle panel). β-actin was the loading control (bottom panel). These blots are representative of 3 experiments and were performed on 2 gels at the same time.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410004.jpeg?Expires=1724917267&Signature=DO2oHlc4NiLBfjORJOWprpiqfevidbBO-voupU-08OqeIxAwjR8Qv8lxt2IuUWAto3ev4DZVXGiWjnjKXeGo6ypqlTgA4NsnqMgctnFrUN8gHY7GLthSTgzMGPMyw9nBUsPS6hk~cFt2jwbT3xgxraus1AT6ENxnvM5alMVZSXIkpHxWfShCLL25m5tQlsUJ56~bgSVAiXcjcq4o~ZXEd1OcpyucW780hPYFeVxAAznE5TTxqklzejGj-OFbF8~17WmZzmfxzUSfFs6awSf7~7~-PwjJZV6t8RIFoEGnzDTGX~7ZteTdFE74I1JDFXn1us5VLBWIehRc3pmADpwJXQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression and function of D156 PCFT mutants. (A) [3H]-MTX influx was performed at 0.5μM and pH 5.5 over 1 minute. Data are the mean ± SEM from 3 independent experiments performed on different days. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein at the cell surface (middle panel). β-actin was the loading control (bottom panel). These blots are representative of 3 experiments and were performed on 2 gels at the same time.
