Figure 6
Figure 6. Thymocyte cells from LckCre+BaxBak DKO mice are resistant to DNA damage-induced apoptotic signals. Thymocytes from LckCre+BaxBak DKO and control mice were treated with 1mM HU, 2 Gy IR, and 10 J UV as indicated. (A) Cell viability was determined by annexin V staining with propidium iodine exclusion followed by flow cytometry at the indicated time points. Representative flow cytometric plots are shown at the 24-hour time point. (B) Quantification of the average cell survival (annexin V negative) from duplicate samples by flow cytometric analysis at the indicated time points. Data are representative of at least 2 independent experiments.

Thymocyte cells from LckCre+BaxBak DKO mice are resistant to DNA damage-induced apoptotic signals. Thymocytes from LckCre+BaxBak DKO and control mice were treated with 1mM HU, 2 Gy IR, and 10 J UV as indicated. (A) Cell viability was determined by annexin V staining with propidium iodine exclusion followed by flow cytometry at the indicated time points. Representative flow cytometric plots are shown at the 24-hour time point. (B) Quantification of the average cell survival (annexin V negative) from duplicate samples by flow cytometric analysis at the indicated time points. Data are representative of at least 2 independent experiments.

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