MDSCs preserve GVL effect of allogeneic donor T cells. Lethally irradiated BALB/c mice were given 10 × 106 B6 T cell–depleted BM cells alone or with 2 × 106 CD25-depleted B6 T cells. Cohorts of mice also received 3 × 105 A20 cells and/or 6 × 106 B6 MDSC-IL-13. All cells were given intravenously on day 0 of transplantation. (A) Kaplan-Meier survival curve of mice receiving BM only (N = 10), BM + T (N = 10), or BM + T + MDSC-IL-13 (N = 10). (B) Corresponding weights from these mice. (C) Kaplan-Meier survival curve of mice receiving BM + A20 (N = 10), BM + T + A20 (N = 10), or BM + T + A20 + MDSC-IL-13 (N = 10). (D) Corresponding weights. (E) Mice were monitored on days 14, 18, and 50 using bioluminescent imaging to detect Renilla luciferase-expressing A20 cells. BM + T versus BM + T + MDSCs, P < .001; BM + A20 versus BM + T + A20, P < .001; BM + T + A20 versus BM + T + A20 + MDSCs, P < .001. Data represent one experiment.
