8-NH₂-Ado treatment of MCL cell lines reduces the rates of macromolecule synthesis. 8-NH₂-Ado inhibited the rates of (A) transcription as quantified by uridine incorporation, (B) DNA synthesis as quantified by thymidine incorporation, and (C) translation as quantified by leucine incorporation. MCL cell lines were treated continuously with 3μM 8-NH₂-Ado for 24 hours. Because 3μM 8-NH₂-Ado was below its EC₅₀ (Figure 1), Mino cells were also treated with 6μM 8-NH₂-Ado for uridine and leucine incorporation experiments. All sample values were normalized to cell count. For [³H]uridine and [³H]thymidine incorporation values, the total counts of the acid-insoluble pellet were also normalized to the specific activity of UTP and TTP in the acid-soluble fraction at the end of the 45-minute chase. Independent experiments were performed in triplicate shown with SD error bars.