Cell death and growth inhibition with 8-NH2-Ado are associated with accumulation of intracellular 8-NH2-ATP and reduction of ATP and UTP concentrations. (A) Three micromolar 8-NH2-Ado accumulated as 8-NH2-ATP and depleted ATP after continuous treatment for up to 24 hours. (B) 8-NH2-ATP accumulation and depletion of ATP was dose-dependent after 24 hours for MCL cell lines JeKo and Granta 519. (C) ATP and UTP pool concentrations were substantially depleted after 24 hours of continuous 3μM 8-NH2-Ado treatment. For panels A through C, experiments were performed with JeKo and Granta 519 cells. (D) Cell death after 24 hours of 8-NH2-Ado treatment at multiple concentrations was positively associated with 8-NH2-ATP:ATP ratios for the 4 MCL cell lines. Independent experiments were performed in triplicate shown with SD error bars.
