Treatment of WT/ITD mice with the FLT3 inhibitor CEP-701 showed an increase in efficiency and a decrease in misrepair frequency and deletion size during NHEJ. Relative repair efficiency (A) and misrepair frequency (B). (C) An agarose gel image of colony PCR products using as templates randomly selected misrepaired products from NHEJ assays. The size of a PCR product from a correctly repaired DSB is 628 bp. PCR products of a smaller molecular weight indicate that deletions have occurred during repair. Molecular size markers are indicated on the right. Vertical lines have been inserted to indicate repositioned gel lanes. (D) Scatter plot showing the distribution of DNA deletion size in randomly selected misrepaired products. P values are shown for all graphs. Vertical lines represent SD. The mean is indicated as a solid horizontal line in scatter plot.