Wild-type and mutant VWF expression in cellular models. (A) Western blot analysis of wild-type and mutant VWF in conditioned media (top panel) and in cell lysates (bottom panels) with the monoclonal antibody pool M31,^22,23  recognizing domains downstream of the breakpoint junction. Migration of the mature wild-type (matureWT-VWF) and deleted (matureDEL-VWF) proteins, and of forms containing the propeptide (proWT-VWF), is indicated. For comparison, see the Western blots in supplemental Figure 4. (B) VWF:Ag (white bars) and VWF:CB (gray bars) levels in conditioned media. The relative molar amount of pWT, pDEL, and vectors and the addition of siRNAs (40nM) are indicated in the table. 100% VWF:Ag corresponds to 445.1 ± 54.5 ng/mL and 100% of VWF:CB to 108.0% ± 14.7% of normal standard. Results from at least 3 independent experiments are reported as mean ± SEM. Statistical significance was evaluated by one-way analysis of variance with Bonferroni posttest: *P < .001; ○P < .01. nd indicates not detectable.