miR-181a is required for cell adhesion–mediated Bim down-regulation and drug resistance, and anti–miR-181a sensitizes lymphoma cells to mitoxantrone or SAHA-mediated apoptosis. (A-D) Jeko-1 cells transfected with anti–miR-181a or anti-miR-control (anti–miR-Ctrl) were treated with vehicle control (VC) or mitoxantrone (0.2μM) or SAHA (0.5μM) for another 24 hours with or without HK cell adhesion. Bim expression levels (A-B) were then analyzed by Western blot, and apoptosis results (C-D) were analyzed by flow cytometry using annexin V. Data are representative of 4 independent experiments with means ± SD. In panels A and B, the relative change in Bim protein was measured by quantitative densotometry and is indicated below each lane.