Figure 1
![Figure 1. Treatment of anti-D with EndoS does not affect the binding of anti-D to RBCs. (A) Western blot analysis of anti-D on RBCs using peroxidase-conjugated anti–human IgG. (B) Glycan hydrolysis of anti-D on RBCs determined by lectin ELISA (optical density [OD] = 450 nm). (C) Quantification of RBC-bound anti-D by ELISA (OD = 450 nm) using antiserum against human IgG.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/24/10.1182_blood-2009-08-239020/4/zh89991053770001.jpeg?Expires=1723405819&Signature=pRQbctHOa24khUzbtru8ba6xOxpFx64dsyV9nhRMSsLt-p6ce-HWbvN4dga2-BhZIq7nV~jBxwnGXp5mEW6S4iz4QUQpRzgWpgkeg6-yzf2eko~jsv2wam41Qezk3k~WqOkS79ZWvOUbnDyyXm-6gnb-XQm0uKJddhV-m~dpv2YXIrqPIjtn-STvMLzRMK-7sjkukMIvQSE0Kpm9v86zrJuaUho-GitgGZqRdU72DbVAbaN8LZQhFMUTrBik5BO0gKASvj9u-HzgMJFBQhqtI2mvEXrzBoOfAfQbE8Rqt5NZuJOoMZiU3NC6Ctn6go2KhqTHJcV8Ru171imcjpbf4Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Treatment of anti-D with EndoS does not affect the binding of anti-D to RBCs. (A) Western blot analysis of anti-D on RBCs using peroxidase-conjugated anti–human IgG. (B) Glycan hydrolysis of anti-D on RBCs determined by lectin ELISA (optical density [OD] = 450 nm). (C) Quantification of RBC-bound anti-D by ELISA (OD = 450 nm) using antiserum against human IgG.
