Figure 1
![Figure 1. Low levels of PDI are expressed on the surface of ECs. Confocal analysis of PDI immunostaining was performed on nonpermeabilized (A) or Triton X-100-permeabilized (B) EA.hy926 cells and compared with an irrelevant rabbit IgG or secondary antibody alone. Scatter plot representation and statistical analysis (one-way analysis of variance [ANOVA]) of MFIs of more than 50 individual cells per experimental condition are shown on the right. (C) Flow cytometric analysis of PDI immunostaining on surface or Triton-permeabilized ECs, shown as MFI ± SEM. ***P < .001, unpaired t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/6/10.1182_blood-2009-10-249607/4/zh89991055410001.jpeg?Expires=1722731291&Signature=2d8bBoidbqigJ9SjFEUBGCuQ4NiWd3jc5FKB2LwAKmSQN53tl5ZEAef14Fm1v54PBgXUiOXxUvQiq766GuVB30hOuwg-joWFIHK95~Qk-4TAPV1LV5O2fr3qmiYhYXsVlP2QSqZ8DPULiCSbjs2Pv8T-otrVHcBhyXhHEb9ciU1wTvLhrUyfgI6CLAT~zLJYhYKVREIAVI6SKFZ9hwtKnz5X~tIjnU3L1lXhFW6RdvgozLaBBTnI2F1ct3H~FyGeDKieyB5YNCPvcgSUpWeUCWDmBQ5sKKPzOlmDk6OrRWczhG2mROQOL8tLGETXqZpuuxhdEpSFb6ps33Bh6j7ngA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Low levels of PDI are expressed on the surface of ECs. Confocal analysis of PDI immunostaining was performed on nonpermeabilized (A) or Triton X-100-permeabilized (B) EA.hy926 cells and compared with an irrelevant rabbit IgG or secondary antibody alone. Scatter plot representation and statistical analysis (one-way analysis of variance [ANOVA]) of MFIs of more than 50 individual cells per experimental condition are shown on the right. (C) Flow cytometric analysis of PDI immunostaining on surface or Triton-permeabilized ECs, shown as MFI ± SEM. ***P < .001, unpaired t test.
