Deficient peripheralization and different repopulation kinetics of human transplants in IL2RG^−/− mice. (A) Very low engraftment levels of human cells at 28 weeks after mPB transplantation indicate quantitative contribution of STRC-ML to hematopoiesis only for the first 5 months (n = 12). Horizontal bars indicate mean values. Data were pooled from 3 independent experiments. (B) LTRCs contribute efficiently to stable long-term engraftment in the BM of IL2RG^−/− mice after CB transplantation (n = 10). Horizontal bars represent mean values. Data were pooled from 3 independent experiments. (C) Similar proportions of human CD45/71⁺ cells in PB and BM of IL2RG^−/− mice transplanted with human CD34⁺ CB cells (n = 10). (D) Proportion of human CD45/71⁺ cells in murine BM at 3 weeks after transplantation of human CD34⁺ mPB cells is significantly higher compared with murine PB (n = 12). (E-F) Deficient peripheralization of human myeloid cells after transplantation of human CB (E) and mPB (F) CD34⁺ cells. GlycA indicates glycophorin-A; and w, weeks. Error bars represent SD. *Significant difference (P < .05).