Human STRC-M and STRC-ML efficiently engraft in IL2RG^−/− mice. (A) Representative fluorescence-activated cell sorter profiles of human hematopoietic lineages in BM of IL2RG^−/− mouse at 3, 8, and 12 weeks after transplantation of 2 × 10⁵ CD34⁺ cells. Percentages in fluorescence-activated cell sorter plots refer to lineage marker-positive cells of 2 × 10⁴ total BM cells analyzed. (B) Human CD34⁺ CB cells show similar multilineage differentiation patterns in the BM of IL2RG^−/− mice (n = 10) and B2m^−/− mice (n = 10) 3, 8, and 12 weeks after transplantation. (C) In IL2RG^−/− mice (n = 12), a larger proportion of CD34⁺ and GlycA⁺ cells were detected at 3 weeks and CD33⁺ cells at 8 weeks after transplantation of human mPB CD34⁺ cells compared with B2m^−/− mice (n = 6). GlycA indicates glycophorin-A; w, weeks; PE, phycoerythrin; and FITC, fluorescein isothiocyanate. Error bars represent SD. *Significant difference (P < .05).