Inhibitory antibodies targeting the major platelet collagen-binding axes reduce thrombus formation on a mixed peptide substrate under flow conditions. A surface consisting of CRP, GFOGER, and VWF-III was perfused for 5 minutes with DiOC₆-labeled whole blood at a shear rate of 1000s⁻¹ after treatment as indicated with the following antibodies: 10B12, 6F1, and RU5, which block GpVI, integrin α₂ subunit, and VWF A3 domain, respectively. (A) Thrombus or platelet surface coverage (%). (B) Mean thrombus height (μm). (C) ZV₅₀ (μm). (D) Representative images showing thrombus surface coverage after treatment with maximal concentration of antibody or in the absence of treatment.