Cytolytic effector function of a murinized Hu gp100(280-288)A2.1-specific scTCR in Hu CD8⁺ T cells. (A) Cytotoxicity of Hu CD8⁺ T cells transduced with Hu WT TCR gp100, Chim scTCR gp100 coexpressed with Mu Cα, Chim scTCR S79C coexpressed with Mu Cα T84C, Hu scTCR gp100 with Mu Cα, and Chim scTCR gp100 with Hu Cα toward peptide-pulsed T2 cells at the indicated CD8⁺:T ratio. (B) Cytolytic recognition mediated by a panel of TCR gp100 constructs as described in panel A toward the Hu gp100⁺A2.1⁺ melanoma cell line Mel526 and as a negative control toward gp100⁺A2.1⁻ Mel397 at the indicated CD8⁺: T ratios. The percentages of lysis are calculated as means from replicates and shown for a representative chromium release assay of 2 experiments.