Figure 3
Expression and tetramer avidity of a murinized Hu gp100(280-288)A2.1-specific scTCR in Hu T lymphocytes. (A) Mock, Hu WT TCR gp100, Hu scTCR gp100/Hu Cα, Chim scTCR gp100, Chim scTCR gp100/Mu Cα, and Chim scTCR gp100 S79C/Mu Cα T84C transduced Hu T cells were tested for binding tetrameric gp100(280-288)A2.1 complexes by flow cytometry. (B) Avidity (KD) of tetramer binding to T cells determined by Scatchard analysis. Hu WT TCR gp100, Chim scTCR gp100/Mu Cα, and Chim scSL7TCR gp100 S79C/Mu Cα T84C transduced Hu CD8+ (○) and CD4+ (●) T lymphocytes were quantitatively tested for binding to tetrameric gp100(280-288)A2.1 complexes by flow cytometry. Linear regression analysis was accomplished with GraphPad Prism Software version 3.0. FITC indicates fluorescein isothiocyanate; and PC5, phycoerythrin-cyanin 5.

Expression and tetramer avidity of a murinized Hu gp100(280-288)A2.1-specific scTCR in Hu T lymphocytes. (A) Mock, Hu WT TCR gp100, Hu scTCR gp100/Hu Cα, Chim scTCR gp100, Chim scTCR gp100/Mu Cα, and Chim scTCR gp100 S79C/Mu Cα T84C transduced Hu T cells were tested for binding tetrameric gp100(280-288)A2.1 complexes by flow cytometry. (B) Avidity (KD) of tetramer binding to T cells determined by Scatchard analysis. Hu WT TCR gp100, Chim scTCR gp100/Mu Cα, and Chim scSL7TCR gp100 S79C/Mu Cα T84C transduced Hu CD8+ (○) and CD4+ (●) T lymphocytes were quantitatively tested for binding to tetrameric gp100(280-288)A2.1 complexes by flow cytometry. Linear regression analysis was accomplished with GraphPad Prism Software version 3.0. FITC indicates fluorescein isothiocyanate; and PC5, phycoerythrin-cyanin 5.

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