Expression of a CD8-independent p53(264-272)A2.1-specific scTCR in Hu T lymphocytes compared with WT TCR-transduced T cells. (A) TCR p53 or MDM2-transduced human T cells (Mu scTCR p53, Mu scTCR p53/Mu Cα, Mu WT TCR p53, Mu WT TCR MDM2) were tested by flow cytometry for expression of Hu CD4 and the Mu subfamily Vβ3 of the p53-specific TCR. MFIs of TCR expression were given for CD4⁺/CD8⁺ T cells. (B) MFIs of p53(264-272)A2.1 tetramer–labeled p53- or MDM2-specific scTCR (Mu scTCR p53, Mu scTCR p53/Mu Cα) and dcTCR (Mu WT TCR p53 or MDM2) transduced Hu CD8⁺ and CD4⁺ T lymphocytes.